You and your partner want to determine the glycogen concentrati homogenates and
ID: 148046 • Letter: Y
Question
You and your partner want to determine the glycogen concentrati homogenates and using the glucose standard curve, you determine the glucose measurement (without amyloglucosidase) and find it to be 0.23 mg/ml, and likewise using the glucose standard curve, you determine the glucose concentration (after amyloglucosidase treatment) and find it to be was 0.94 mg/ml. From these values you estimate the glycogen concentration to be 1.42 mg/ ml glucose released from glycogen. Your partner estimates the glycogen concentration to be 0.71 mg/ml glucose released from glycogen. Which one of the two values is correct and what was forgotten in the calculations to give the incorrect answer? on of one of the tissue 4. 5. Propose a reason why glucose oxidase reacts with glucose, and not glycogen.Explanation / Answer
The correct value is 0.71mg/ml.
Justification
Glycogen content is calculated as difference between the glucose content in absence and presence of enzyme amyloglucosidase.
In the given problem, the difference is 0.94 - 0.23 = 0.71
The error in other result is "multiplication by 2" which may probably occurred due to incorrect use of dilution factor
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