With reference to the metabolic pathways involved, and the resultant energy yiel
ID: 147470 • Letter: W
Question
With reference to the metabolic pathways involved, and the resultant energy yield, explain your observations of growth time across the strains under the different conditions:
Concentration of Extracellular Glucose in growth medium Growth time of cultures Cell concentration cells/mL rowth (Tm 3.75 mM No. Carbon O2 Strain of EtOH Presence at cessation of Initial (To)Final (Tm) source 30 1Glucose 8.450 0.022 0 20 Yes 0.35 8.399 0.017 20 2 Glucose Yes 0.55 8.637 0.0189 20 Yes 0.5 8.688 0.0183 30 Pyruvate 5.2 NIA NIA 0 NIA NIA NIA 0Explanation / Answer
From the observation table provided we can conclude that for strain Gr the growth time of culture is 30 hours in the presence of glucose, pyruvate, and absence of EtOH at a maximum cell concentration of 5.2 cells/ml. However, in absence of glucose source and presence of EtOH growth time is reduced to 20 hr. In case of strain Pe, we observe that both presence or absence of glucose does not affect the growth of the cell as long as EtOH is present the Growth time of the culture reduces to 20 hr. Hence it can be concluded that strain Pe is using EtOH as the carbon source for metabolism. whereas strain Gr does not utilize EtOH as a carbon source.
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