molecules in a cell, what would be the best form of light microscopy to use? A.
ID: 147448 • Letter: M
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molecules in a cell, what would be the best form of light microscopy to use? A. Nomarski B.phase-contrast C.brightfield, unstained D.brightfield, stained .fluorescence 15 Ifyou were asked to use a light microscope to locate specific 16 Which is not part of a prokaryotic cell? A.nucleoid B.chloroplast C.ribosome D.plasma membrane E.cell wall 17 Which is not part of a eukaryotic cell? A.nucleoid B.chloroplast C. ribosome D.plasma membrane E.peroxisome Ifyou fluorescently labeled a protein and found it ended up in the nucleus, through what must it have passed? 18 A.pore complex B.nucleolus .chromatin D.endoplasmic reticulum E.not necessarily any of these Where would you place an arrow on a diagram of a cell if the arrow was to point to the place where cytoplasmic proteins are made? 19Explanation / Answer
15. Option E is the answer, fluorescence microscopy is very powerful and precise to locate the biomolecules. In this technique we use antibodies coupled with fluorescent dyes. When antibodies bind up with targated molecules complex gives fluorescence that can be recorded and suggests the location of targated molecules.
16. Answer is B. Chloroplasts are found in plant cells which are eukaryotic cells. In prokaryotes chlorophyll molecules are found. Chloroplasts themselves are the evolutionary modification of prokaryotes and have endosymbiotic association with eukaryotic cells (plant cells).
17. Answer is A. As prokaryotes don't have exact nuclear oundaries, the genome or genetic material of prokaryotes is irregular. This irregular structure of genetic material is called nucleoid. That's why answer is nucleoid is absent in eukaryotic cells.
18. Answer is A. Fluorescent labelled protein molecules which are targated to the nucleus must go through the nuclear pore complex. Because NPCs (Nuclear pore complexes) are the only way to exit or enter to the nucleus.
19. Most of the proteins destined for secreation are syntgesyzed in Rough ER, in contrast Cytoplasm destined proteins are synthesized in free ribosomes. Thats why arrow will be on Free Ribosomes.
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