TABLE 2-4.Results from Biurot Test PEPTIDE 8OND PRESENT (r) OR ABSENT ) SAMPLE I
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Question
TABLE 2-4.Results from Biurot Test PEPTIDE 8OND PRESENT (r) OR ABSENT ) SAMPLE INITIAL COLOR FINAL COLOR clear clear cleaf cleor cleaf cleal etear clear cleal elear clear clooAy Alarine Blve Blve 2. Arginine Purple 4. Cosein 5. Cysteine 6 Glutamic acid 7. Glycino Black Blue Blue Blue Blue Blve BlUe Blue Blue Hydrolyzed casoin 9 Proine 10. Tyrosine 1 Urea 12) Unknown A 13. Unknown 8 14. Waler For your lab report Were your predictions coret? If theve ave disorepancies betueen your predictions and your results, how might these be explained? NOTE: Examples of chromatograms and table of R, values for selected omino ocids in conditions (chro- matography solvent and stationary phase) that match our conditions:htp://www.reachdevices.com/ TLC aminoacids.html Using the colors and Ry values for the known samples, try to determine the identity of the amino acids (if any) in the hydrolyzed casein, unknown A, and unknown B. Which substances reacted with biuret reagent? Why? Which substances did not react with biuret reagent? Why not? Did the eslts for each test agree with your predictions? Explain any disrepancies WORKSHEExplanation / Answer
Only biuret reacted with biuret reagent and formed purple colour. In the presence of peptides, a copper(II) ion forms violet-colored coordination complexes in an alkaline solution. Due to absence of peptide bond, remaining substances didnot form purple colour.
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