1.5 A. Multiple Choices questions (for each question, only one choice is correct

Question

1.5 A. Multiple Choices questions (for each question, only one choice is correct. point/question, 9 points in total) 1) During transcription newly synthesized RNA forms base pairing with coding sequenco a. for short distance b. for whole length for no length d. half length. 2) Each DNA has how many reading frames? a. b. 2 c. 3) Melting of DNA/RNA involves disruption of the following Hydrogen bonds between pairing bases b disruption of hydrophobic interactions between stacking bases c. none d. both. 4) Different replicons in bacterial cells can be a. Plasmid DNA b. Bacterial chromosome c. Phage DNA d, all 5) Many proteins involved in genome expression are in complex form called a. primary structure b. secondary structure c. tertiary structure d. quaternary structure 6) Based on the method to estimate Tm temperature of primer-template hybrid according to the GC and AT nucleotide number of a primer (we discussed this in lecture 3 PCR), what is the best annealing temperature when use the primer 5'-TTGCTTCCATGTGCTCCAGA- 3' for doing PCR? a. 51 °C b. 65°C c. 57 C d. 42 °C e 72 °C

Explanation / Answer

1. Option C is correct.

As RNA does not form base-pairs. Only DNA does.

2. Option D is correct.

Each DNA has 6 reading frames.

Each strand consists of codons of 3 amino acids which can be read in 3 ways and there are 2 strands.

so, 3*2= 6

3. Option A is correct.

Melting causes breaking of hydrogen bonds, which can be reformed if cooled.

4. Option D is correct.

All of the above can replicate in bacterial cells.

5. Option D is correct.

Quarternary structure of proteins is a complex form consisting of more than one peptide.

They are involved in genome expression.

6. Tm temperature (approx.)= 2*(A+T) + 4*(G+C)

= 2*10 + 4*10 (counting)

= 20 + 40

= 60 C

The required temperature is about 5C lower than this.

so option C would be correct as it is near 55 C

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