Which of the following statements is true of the Western Blot? Select any/all an
ID: 147038 • Letter: W
Question
Which of the following statements is true of the Western Blot? Select any/all answers that apply.
A.
A Western Blot is commonly used prior to running SDS-PAGE.
B.
A Western Blot allows us to see all the proteins in a given sample.
C.
A Western Blot exploits antibodies in order to see a particular protein in a given sample.
D.
A Western Blot is commonly used as a protein purification step.
E.
A Western Blot is one common technique used after SDS-PAGE has been run.
A.
A Western Blot is commonly used prior to running SDS-PAGE.
B.
A Western Blot allows us to see all the proteins in a given sample.
C.
A Western Blot exploits antibodies in order to see a particular protein in a given sample.
D.
A Western Blot is commonly used as a protein purification step.
E.
A Western Blot is one common technique used after SDS-PAGE has been run.
Explanation / Answer
The correct options are:-
C. A Western Blot exploits antibodies in order to see a particular protein in a given sample.
E. A Western Blot is one common technique used after SDS-PAGE has been run.
Western Blotting is done only after doing the SDS PAGE. The proteins are first separated on SDS PAGE and then the bands are transferred to nitrocellulose membrane to detect the presence of a particular protein of interest with the help of a primary antibody against that protein. The primary antibody is then detected using a secondary antibody which is finally detected through chemoluminescense. That is the whole process of Western Blotting.
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