Q 2. Fumarase catalyzes the hydration of fumarate (1) to L-maleate (2). This hyd

Question

Q 2. Fumarase catalyzes the hydration of fumarate (1) to L-maleate (2). This hydration reaction has a Vmax of 5.1x103 M min-1 and a Km of 2.0x103. Kinetic data for the inhibitor citrate (3) are shown below in the table H20 Km-2.0x103 M CO2 fumarase . 5.1x103 M min fumarate (1) L-maleate (2) e citrate (3) Co2 nhibitor) 02C citrate conc.(10-3 M) Vmax (10-3 M min-1) Km(10-3 M) 0.0 3.5 7.0 Given the Lineweaver-Burk plots above for fumarase with three concentrations of citrate-0.0, 3.5, and 7.0x10 M What type of inhibitor is citrate for fumarase? Based on the structure of citrate, is this reasonable? Assume that compound 4 was found to also be an inhibitor of fumarase? Without knowing the kinetic data, what type of inhibitor might you expect 4 to be (or not 5.1 5.1 5.1 0.5 1.0 2.0 H3N be)? inhibior

Explanation / Answer

1)CITRATE IS a competitive inhibitor as they only affect km value by interfering with the binding of substrate and do not change vmax value as it cannot bind to ES complex.

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