1. Assessing accuracy and proficiency: Do you get the same result if you measure
ID: 146890 • Letter: 1
Question
1. Assessing accuracy and proficiency: Do you get the same result if you measure the same dilution tube twice? What are some reasons you might not get the same measurement? 2. Does this OD reading tell you how many live cells are in culture? What advantages are there to measuring cells with the s What disadvantages? 3. How confident are you that you accurately identified the concentration of pigment? List three caveats to your conclusion about the concentration of pigment in your spent medium: 4. If you isolated a bacterium from nature or from a patient and it produced a soluble pigment, how cculd you characterize it?Explanation / Answer
1. No, we will not get the same result if we use the same dilution tube twice.
The reason is that : some solution might get stick with the tube which is not completely washed away, so the solution of first attempt can interfere in the results of the second attempt.
2. No, it doesn't tell us about the amount of living or non living bacteria. Only the total number of bacteria are identified.
The reason lies in the principle used by the spectrophotometer which is that; it measures the light absorbed by a substance coming in the path of light.
So we cannot distinguish whether the bacteria is alive or dead.
Adv. : It is OD method which is quite simple and requires less technique.
Also it is an accurate method for calculating OD.
Disadv. : We cannot distinguish between live and dead bacteria.
3.
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