cm ced on and the amount of gas produ Produce a graph for all three tubes with t
ID: 146734 • Letter: C
Question
cm ced on and the amount of gas produ Produce a graph for all three tubes with the pH ranges on the x-axis, the y-axis. Use your results to explain the relationship that pH has on enzyme a ctivity Experimental Procedure: Effect of temperature on Catalase Ac tivity talase. Before runni This experimental procedure is to test the effects of temperature on the activity of ca the experiment, be sure to state your hypothesis describing the effect of temperature on Mark and label four clean test tubes. Tube l: 1. Add the catalase solution to the 1 cm mark. ng catalase activity 2 2. Fill to the 8 cm mark with distilled water. 0 3. Place in ice bath for 15 minutes. Add hydrogen peroxide to the top of the small tube. Place large tube on over the top of the small tube and invert. Measure the production of gas produced in the tube for a total of 20 minutes and record distance moved in Table 5.2 4. 5. 6. 6 Laboratory 5 Enzyme Activity Lab 54Explanation / Answer
Hi,
To draw the graph, you will need the values. I assume you have it and will explain how to use them.
1. Problem- Is enzyme activity depends on the temperature of the solution.
2. Enzymes act on specific substrates and convert them into products. The enzymes can fasten the rate of reaction bybreducing the activation energy. The enzymes have an active site where the substrates are converted to products. Enzyme activity generally depends on several factors such as substrate concentration, product concentration, temperature, pH, presence not inhibitors. The activation energy is lowered by binding of enzyme to substrate and thus makes the products faster. It does not change the eauiliequil but only attains it faster.
In this experiment, we assessed the role of temperature in catalase enzyme activity. Catalase is an enzyme which can detoxify the Hydrogen peroxide into water and oxygen. It is an antioxidant.
IndelendInd variable: enzyme Concentration,
Dependent variable: evolution of gas bubbles, temperature.
The Hydrogen peroxide was added to solution of catalase enzyme. The enzyme produced the oxygen gas which was determined by the observer. The temperature of the set up was kept at 4 degree Celsius. Another expt was done by imcreasini the remptempera to 35C. The gas formation in both the cases is monitored and plotted on a graph.
The gas formation was found to increase with the rise in temperature. At 37 C the catalase activity was the highest. Cold inhibits the enzyme activity.
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