16. Empty magnification occurs when (A) a microscope without lenses is used. (B)
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Question
16.Empty magnification occurs when
(A) a microscope without lenses is used.
(B) the size of an image increases with no increase in resolution.
(C) the size of an image increases with an increase in resolution.
(D) the resolution of an image increases while the size of the image decreases.
(E) the resolution of an image decreases while the size of the image remains the same.
17.Which of the following is closest to the resolution limit of a light microscope?
(A) 2x10-5m (B) 2x10-6m
(C) 2x10-7m (D) 2x10-8m
(E) 2x10-9m
18.A specimen has a diameter of 2 m. What is the minimum magnification that will allow a human to resolve this object?
(A) 5X (B) 10X
(C) 50X (D) 100X
(E) 500X
19.When magnifying an image with a lens, which of the following interactions of light with matter is most critical?
(A) absorption (B) reflection
(C) refraction (D) scattering
(E) interference
20.The ability to visualize a specimen through a light microscope is NOT improved by
(A) decreasing the numerical aperture (B) the use of immersion oil
(C) staining the specimen to improve contrast (D) using shorter wavelengths of light
(E) using lens with wider angle of aperture
21.In fluorescence microscopy, the incident light that is absorbed by the specimen has ________________ energy and _____________ wavelength compared to the fluorescence emission.
(A) lower, longer (B) lower, shorter
(C) higher, longer (D) higher, shorter
(E) higher, similar
22.In a compound microscope, light travels from the light source to the eye through the following order of lenses:
(A) condenser, objective, ocular (B) ocular, condenser, objective
(C) objective, condenser, ocular (D) objective, ocular, condenser
(E) ocular, objective, condenser
23.Which of the following is an advantage of electron microscopy?
(A) Living specimens can be observed. (B) The microscope is inexpensive and easy to use.
(C) Specimens can be observed at high resolution. (D) No artifacts are produced.
(E) All of the above.
24.Observations of bacterial flagella during motility are best suited to
(A) bright-field microscopy (B) dark-field microscopy
(C) SEM (D) TEM
(E) NMR
25.The best microscopy technique for observing a section through the different layers of the bacterial cell envelope is
(A) SEM (B) TEM
(C) phase contrast microscopy (D) atomic force microscopy
(E) dark-field microscopy
Explanation / Answer
16 b(size of image increases with increase in resolution)
17. b
18. d (100X)
19. c (refraction)
20. a (decreasing numerical aperture)
21. a (lower, longer)
22. a (condenser, objective, ocular)
23. c (specimens can be observed at high resolution)
24. (dark field microsscopy)
25. b (TEM)
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