This question is asking you to come up with the recombinant plasmid map based on

Question

This question is asking you to come up with the recombinant plasmid map based on the evidence in the gel image and the maps given to you. I am especially confused on how cutting the DNA with Kpn1 gives you a kb higher than what the DNA was to start with. If you could help me figure this problem out, or at least get me started, I would really appreciate it.

STAPLES ucose is required to prepare 1.5 L of a 5% w/v solution? 8. (2 pts) What ma In vitro (in the test tube) a plasmid exists in multiple forms as discussed in the laboratory lecture. Despite having the same molecular mass the three different for move at different velocities during electrophoresis. Below is a depiction of ~0.8% agarose gel 9. C. crassicaudata Experiments (3-7) 7.0 5.0 3.0 2 3.0 tn 1.5 0.7 0.5 0.3] 0.11 (2 pts). From Lane 2 identify the three forms of the plasmid DNA Linear Supercolied C. 2 3. Circular (2 pts), Given the data in lane 2 of gel above what is the approximate size of the plasmid now named p2484? 3 Wb 10 (6 pts). A student from Biochemistry 2484 has volunteered to work in a laboratory which is describing interesting genes in the Amazon's superstinger wasp Calistoga crassicaudata. C. crassicaudata DNA has been ligated into the multiple cloning site (MCS) of p2484. Unfortunately, the student did NOT follow Dr. Reilly's admonition to label, label, label and

Explanation / Answer

Ok i can understand your problem and we can easily understand it.

First we need to known about the enzyme kpn1 it is a endonuclease that make a blurnt end and work on a fix 6 nucleotide sequence that sequence is GGTACC and cut between C*C .

If we can calculate the possibility of the sequences in the normal nucleotide sequences.

If the are the enzyme may be specific for only one nucleotide then it cut after 4 nucleotides because there are 4 type of base in the DNA. Then we can say 4¹=4

If there may be 2 nucleotide in the cutting sequence of enzyme it cut after 16 base (brcabec it's possibility after 16 base) 4²=16

But there are 6 nucleotide in sequences and the same sequences exist in same DNA strand is about 4^6 nucleotide and it is equal to 4096 base pairs.

So we can say that knp1 cut after approximately more than 1K base pair.

I think you can understand thank you.

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