1. The alpha helix of a protein is on the surface of a protein and is part of a

Question

1. The alpha helix of a protein is on the surface of a protein and is part of a glucose binding site. The helix 20 amino acids to span the length of the glucose molecule. Using a helix wheel, design a 20 amino acid sequence for the helix. Please number your amino acids, so it is clear what order they are in on the helix.

2. a. Draw the structure (at physiological pH) of the peptide WRAPS (note that this is one letter amino acid code).

b. Draw the titration curve for this peptide. Make sure to label the axes and identify the pKa’s and pI.

c. What is the pI of this peptide? d. Determine the charge at pH 2, 7 and 10.

d. Determine the charge at pH 2, 7 and 10.

Explanation / Answer

Sequence A_T

C_G is the sequence of amino acids in the protein Glucose on the helix

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