After growing 3-day static cultures of Pseudomonas fluorescens, you make serial

Question

After growing 3-day static cultures of Pseudomonas fluorescens, you make serial dilutions and plate samples of those dilutions on King's B plates. After incubating the plates at 28 degrees C for two days you find that your plates have too many Pseudomonas colonies on them to count. Which of the following is NOT a potential explanation for this problem? media and cell suspension The same pipette tip used to sample the culture was used for making all of the serial dilutions. The Pseudomonas cultures weren't vortexed sufficiently before sampling. O The King's B plates were made incorrectly. Each dilution wasn't well mixed before removing a sample to make the next serial dilution.

Explanation / Answer

Answer: The same pipette tip used to sample the culture was used for making all the serial dilutions.This is because it doesnot matter as dilutions were made in fresh medium. The more number of colonies are due to high amounts of bacteria present in the sample and thus it needed to be dilute further.

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