After plating samples from a 3-day static culture, you have approximately 6 ml o

Question

After plating samples from a 3-day static culture, you have approximately 6 ml of leftover culture in a glass culture tube. How should you discard the remaining culture and the tube? Leave the leftover culture in the fridge so you can plate samples again 2 days later. Pour the leftover culture down the drain and discard the glass culture tube in a glass waste container. Put the glass culture tube containing the leftover culture in an autoclave waste bag, so it will be put through a kill cycle and discarded. Pour the leftover culture down the drain, rinse the glass culture tube with tap water, and place the tube in the bin next to the sink so it will get washed and reused O Place the tube containing the leftover culture in a rack with other materials that need to be autoclaved, so it will be put through a kill cycle and the tube can be washed and reused.

Explanation / Answer

The correct answer is option C that is put the glass culture tube containing the left over culture in an autoclave waste bag, so it will be put through a kill and cycle and discarded.

There two different cycle for sterilization and killing the waste culture.

Sterilization is done at 121°C for 15-20 minutes and killing cycle is done at 121° for 45 minutes.

If we keep waste with other autoclave material, other media components will get denatured because of prolonged time.

Culture can't be discarded in sink and if we put it in a refrigerator, nutrients will get depleted and culture will die. So, option A, B, D ane E are incorrect.

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