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of the unknown samples (\"UNKI\" \"UNK2,\" and \"L\"). Make your calculations fo

ID: 145865 • Letter: O

Question

of the unknown samples ("UNKI" "UNK2," and "L"). Make your calculations for 150 L per exactly 15 uL. Prepare the dilutions in your appropriately labeled 1.5 mL microcentrifuge tubes. Tube LabelFinalVolume and Type of DiluentSource of Unknown Volume of tube (volume before transfer to next tube). You will receive unknown aliquots measuring Dilution L (undiluted) UNKI (UNK2) undiluted UNKI (UNK2, L) after 1->10 dilution UNKI (UNK2, L) 20 Unknown | 15 ul. uL uL PBS UNK1 PBS + 0.1% TX100 | K2 UNK1 UNK2, L) 20 UNK1 UNK2, L) 40 UNK1 UNK2, L) 80 20 40 1-80 nL PBS + 0.1% TX100 | L PBS + 0.1% TX 100 | L PBS 0.1% TX 100 | UNKI (UNK2, L) 40

Explanation / Answer

Tube label Volume and type of diluent Volume of unknown L 150 ul PBS 15 ul UNK1 (UNK2) 149 ul PBS + 0.1% TX100 15 ul UNK1 (UNK2, L) 20 298 ul PBS + 0.1% TX100 30 ul UNK1 (UNK2, L) 40 596 ul PBS + 0.1% TX100 60 ul UNK1 (UNK2,vL) 80 1188 ul PBS + 0.1% TX100 1200 ul