1. If a specimen is viewed using a 45X objective in a microscope with a 15X eyep
ID: 145634 • Letter: 1
Question
1. If a specimen is viewed using a 45X objective in a microscope with a 15X eyepiece, how
many times has the image been magnified
2. Would you use phase contrast microscopy to view a stained preparation? Would you use a
coverslip on a stained preparation?
3. Would you use bright field microscopy to view a wet mount preparation? Would you use a
coverslip on a wet mount preparation?
4. What is the position on the condenser wheel for (1) bright field – low 10X lens, (2) phase
contrast – high dry (40X) lens, (3) phase contrast – oil immersion (100X) lens, and (4) bright
field – oil immersion (100X) lens?
Explanation / Answer
1)when the objective is 45X and eyepiece is 15X the magnification is 45x*15x=675 the magnification is a product of both objective and eyepiece.
2)to observe a stained specimen the phrase microscope background should always be grey .phase microscope offers possibility to use phase shift s cause by difference s in optical path length.ehich helps to visualize a specimen.The optical path depends on specimen thickness and refractive index.fluoroscence microscopy. Chromatophore is attached to specimen which emits fluoroscence .
No I cannot use a coverslip as it may change the refractive index.
if u need more explaination let me know
3)dark field microscope can be used to observe wet mount.used in motility test
In wet mount we should use coverslip, without that surface tension present may cause the droplet to bunch of in form of a dome .coverslip omits the surface tension and flatten s the specimen.
4)position of condenser wheel on for
1) bright field low 10 x condenser is wheel lowered requires less light
2)phase contrast higj dry 40X
3) phase contrast oil immersion 100x =raised it requires more light
5) bright field 100x raised wheel is raised as it requires more light
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