ert Format Tools Table Window Help , 91% CO Sun 11:47 PM MA D 8 e Lab 3 Microsco
ID: 145503 • Letter: E
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ert Format Tools Table Window Help , 91% CO Sun 11:47 PM MA D 8 e Lab 3 Microscopy and Celll Counting-Saved to my Mac Q Search in C Layout References Mailings Review View x x A.AE. Every objective has a plane of focus (or focal plane), which is a certain distance away from the objective lens where a specimen appears in sharp focus. This plane of focus is characteristic of the lens and cannot be changed. Complete the following sentence: 18. If a microscope is parfocal the plane of focus for each of its objectives are 19. Describe how the plane of focus is related to using the focus knobs to bring a specimen into focus Depth of field 20. Obtain a slide of three crossed strings and focus at the point where the strings cross with your 4x objective 0 5 80Explanation / Answer
18. If a microsope is perfocal the plane of focus for each objectives are fixed or constant
In perfocal microsope the focus the objective remain constant or fixed and while the magnificaiton is changing. For example, if we go for lower to higher magnificaiton like from 10X to 40X or 40X to 10X, for an object, the object will remain in focus and we don't have to adjust the focus each time.
19. there are two types of adjustment knobs in microscope, a coarse and a fine. These two types of knobs are used to adjust the plane of focus so the specimen or object becomes focused and we can see it. Coarse adjust knob is used to move the specimen towards or away from the objective lense. Thus with the help of the coarse adjust knob we can roughly fix the focus plane. Further we can fine tune the focus with the help of fine tune knob.
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