2. In the discussion of this hypothetical lab report you find the following: \"T

Question

2. In the discussion of this hypothetical lab report you find the following: "The parallels between activity and disulfide bonds confirmed previous findings by Herald and Miller" According to Knisely, what is missing from this sentence? Where did you find support for your answer in Knisely? Fix the sentence. a. "The percentage of disulfide bonds and activity increases, which explains its dependence on reaction conditions." According to Knisely, why is this sentence written incorrectly? Where did you find support for your answer in Knisely? b. In the materials and methods section you find: "The absorbance of the tubes were measured from 200 nm to 300 nm." According to Knisely, why is this sentence written incorrectly? Where did you find support for your answer in Knisely? Fix the sentence. c. 3. Look at Figure B. Are the axes drawn in the correct positions according to Knisely? On what page in Knisely do you find support for your answer? Figure B Black activity Gray disulfide bonds Sample A Sample B SSample C 250 270 290 Wavelength (nm)

Explanation / Answer

2-

a- The activity and the disulfide bonds are associated with proteins. The aromatic proteins shows maximum absorption at an absorption spectra at 260 nm. The graph also shows peak at 260-270 nm, this indicates that the protein contains aromatic amino acids in its structure.

Activity means the stabilizing property of a protein structure. Hence in the graph the disulfide bonds and activity show parallel orientation.

b- The increase in the activity with the increase in the disulfide bond is shown. The proteins contain peptide bonds ( bonds between two amino acids) and aromatic amino acids ( tryptophane, tyrosine and phenylalanine). By looking at the graph it can be said that the protein contains trypophan amino acids hence it shows maximum absorption spectra at 270-280 nm along with increased activity.

C- peptide bonds in the proteins show activity at 220 nm. And the lower activity ( fall in peak) indicates non- aromatic amino acids in the protein chain.

3-

The axis drawn are correct the absorbance is shown on the y axis and wavelength on the x axis.

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