Question 1. You are sequencing a 350bp lizard gene using the Sanger dideoxy sequ

Question

Question 1. You are sequencing a 350bp lizard gene using the Sanger dideoxy sequencing method. You have amplified the region using the primer 5'-ATCCGGGCG-3'. What will be the length of the lowest band in your sequencing gel?

(A) Cannot answer the question with the provided information

(B) 350

(C) 10

(D) 9

(E) 351

Question 3. Which of the following answer(s) are TRUE (select all that apply)?

- PCR results in exclusively the desired region to be amplified

. - PCR primers must be outside the region of interest

. - PCR primers must be flanking the region of interest.

- Sequencing primers must be flanking the region of interest.

- All of the nucleotides used in Sanger Sequencing would also be able to be used for a PCR reaction where you are trying to amplify a gene of interest

. - Sanger sequencing requires dNTPs.

- A standard PCR reaction requires ddNTPs

- Small DNA fragments on a gel would be closer to the wells on a gel than larger DNA fragments.

Explanation / Answer

Question1

(A) Cannot answer the question with the provided information

This is because we required both forward and reverse primer sequence in order to state the correct product size in the gel.

Question 3:

Answer TRUE:

Reasons

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