You try it: design two oligos to amplify the histone H3 gene from genomic DNA th

Question

You try it: design two oligos to amplify the histone H3 gene from genomic DNA that would allow the PCR product to be ligated into HindIII (AAGCTT) and BamHI (GGATCC) sites Histone H3 genomic sequence (from 5' end) ATGGCCCGCACTAAGCAGACCGCCCGCAAGTCCACCGGTGGCAAGGCCCCCCGTAAGCAGCTCGCTTCCAAGGC TGCCCGCAAGTCCGCCCCCTCCACCGGCGGTGTCAAGAAGCCCCACCGTTACAAGCCCGGTACCGTCGCTCTCC GTGAGATTCGTCGCTACCAGAAGTCCACTGAGCTTCTGATCCGCAAGCTCCCCTTCCAGCGTCTCGTCCGTGAG ATTGCCCAGGACTTCAAGTCCGACCTCCGCTTCCAGAGCTCTGCCATCGGCGCTCTCCAGGAGTCCGTCGAGTC TTACCTCGTCTCTCTCTTCGAGGACACCAACCTCTGCGCTATCCACGCTAAGCGTGTCACCATCCAGAGCAAGG ACATCCAGCTCGCCCGCCGCCTCCGCGGTGAGCGCAACTAA

Explanation / Answer

The primers would look like this:

Forward primer

5' AAGCTTATGGCCCGCACTAA 3'

Reverse primer

5' GGATCC TTAGTTGCGCTCAC 3'

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