Question: As a recent cell biology graduate, you have just been hired by a biote

Question

Question: As a recent cell biology graduate, you have just been hired by a biotechnology company to develop a biofuel using algal cells that produce an oil very similar to diesel fuel. What model organism(s) might you use for each of the following aspects of this project? What tools and techniques would you use? Determining which genes and enzymes are required to produce the oil.

Fill in the blank: Since the oil is being produced in algal cells, you might try to use ( a ), an ( b ) model organism that is easy to grow in the lab. You could test whether ( c ) produces a similar compound and try to isolate the genes and ( d ) necessary to produce it. Or you could perhaps ( e ) (knock out) genes in your ( f ) cell and see which ones are needed to produce the ( g ).

Options to go in the ( ) blanks are:

1. mutagenize

2. Neurospora

3. Rhodopseudomonas

4. fungi

5. biofuel

6. Chlamydomonas

7. enzymes

8. algal

9. bacteria

10. lipids

Explanation / Answer

Because of u are studying a natural process from algal cells,that DNA that contains the gene that produces proteins that in turn make the biofuel are found in algal cells. Therefore the experiments that would use algal cells to isolate the genes for proteins expected to be part of process. This would be done using the PCR to amplify the genes from algal genomic DNA

E-mutagenize

A,b-neurospora and

rhodopseudomonas

C-algal

D-chlamydomonas

F-bacteria

G-biofuel

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