1. What is the difference between maghification and resolution? What is the maxi
ID: 143626 • Letter: 1
Question
1. What is the difference between maghification and resolution? What is the maximum magnification and resolution normally achievable with a compound light microscope? 2. 3. How does the wavelength of light affect the resolution of a microscope? 4. Why is immersion oil used with certain high magnification objectives? If you examine a sample with a 40X objective lens and a 10X ocular lens, what is the total magnification? 6 What is the purpose of the condenser lens? 7. hat is the difference between a positive stain and a negative stain? 8 What is the advantage of using a microscope with phase-contrast optics compared to 9 hat is the advantage of using a microscope with dark-field optics compared to 10 hat is the purpose of calibrating an ocular micrometer with a stage micrometer? a microscope with bright-field optics? a microscope with bright-field optics?Explanation / Answer
Ans 1: Difference between magnification and resolution
1: Maginification refers to the ability of the instrument to enlarge the size of the image as compared to the original size of the viewed object. Resolution refers to the power of the optical instrument to differentiate detween two closely placed objects.
2: Magnification of a microscope is product of maginification of objective lens and eye piece. Resolution depends on the wavelength of light and the numerical aperture of the lens of microscope.
3: Maginification is the ability of the instrument to enlarge the image while its resolution serves to make the image more clear.
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