1. (8 points) During lab, you used a set of BSA standards with known concentrati
ID: 143331 • Letter: 1
Question
1. (8 points) During lab, you used a set of BSA standards with known concentration by diluting a 5 mg/ml)jtock solution of BSA. Calculate the final concentration of BSA in the problems below using the formula where C1 and V1 are the concentration and volume of the starting solution, and C2 and V2 are the concentration and volume of the final diluted solution. Include proper units and show your work for full credit. A. 300 ul of 5 mg/ml BSA is added to 700 ul TBS B. 50 ulof 1.5 mg/ml BSA is mixed with 450 ul H20 and 500 ul Bradford Reagent C. 10 ul of 1 mg/ml BSA is mixed with 990 ul TBS. lOut ggou D. 10 ul of 0.1 mg/ml BSA is mixed with 990 ul TBS. 1D ot AOU Page 1 of 3Explanation / Answer
A) C1= 5mg/ml
V1= 300ul =0.3ml
C2= ?
V2= (300+700) =1000ul =1ml
So, V1C1=V2C2
0.3X5=1XC2
C2= 1.5mg/ml
B) C1= 1.5mg/ml
V1= 50ul =0.05ml
C2= ?
V2= (50+450++500) =1000ul =1ml
So, V1C1=V2C2
0.05X1.5=1XC2
C2= 0.075mg/ml
C) C1= 1mg/ml
V1= 10ul =0.01ml
C2= ?
V2= (10+990) =1000ul =1ml
So, V1C1=V2C2
0.01X1=1XC2
C2= 0.01mg/ml
D) C1= 0.1mg/ml
V1= 10ul =0.01ml
C2= ?
V2= (10+990) =1000ul =1ml
So, V1C1=V2C2
0.01X0.1=1XC2
C2= 0.001mg/ml
If you have more than 4 queries, please ask in a separate question.
Related Questions
Navigate
Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.