https://www.ets 3. Eukaryotie cells and their organelles are disrupted (E) Outsi
ID: 142994 • Letter: H
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https://www.ets 3. Eukaryotie cells and their organelles are disrupted (E) Outside the cell by sonication. A centrifuge is used to separate soluble and insoluble components. Protein X is found in the insoluble fraction following centrifugation. The insoluble fraction is treated 7. A unique property of lysosomal proteins is that they with 0.3 M NaCT and centrifugation is repeated Protein X is now found in the soluble traction Protein X would be best described as (A) contain a stop-transfer sequence (B) operate only in an alkaline environment (C) are enclosed in clathrin-coated vesicles D) are phosphorylated on mannose residues (E) are modified by O-linked glycosylation of (A) an integral plasma membrane protein (B) an integral membrane protein in an organelle C) a peripheral membrane proteirn (D) a soluble cytoplasmic protein asparagine residues 8. The beadlike unit of chromatin structure is the (E) a soluble nuclear protein (A) chromatid (B) nucleosome (C) kinetochore (D) solenoid (E) scaffold 4. Which of the following macromolecules would d only one type of monomer after complete (A) DNA (B) Glycogen 9. Inactivation of one X chromosome in female mammals is an example of which of the following (D) RNA (E) Triacylglycerol (A) Epigenetics (B) Sex-linked inheritance (C) Autosomal dominance (D) Translational control first exon 16. Ribulose 1.5-bisphosphate carboxylase, which catalyzes the fixation of carbon dioxide during photosynthesis in plants and many algae, is located in which of the following cellular 13. The migration of a protein on an SDS polyacrylamide gel is best described as inversely proportional to the (A) negative charge (B) isoelectric point (C) log of carbohydrate content (D) log of molecular weight (E) native volume (A) Peroxisomes (B) Chloroplasts (C) Mitochondria (D) Lysosomes (E) Plant cell vacuolesExplanation / Answer
3-c) a peripheral membrane protein
4-b) glycogen
Glycogen is a form of energy storage in animals. It is a multibranched polysaccharide of glucose which is made up of alpha-glucose monomers. This glucose is linked together by alpha(1-4) and alpha(1-6) glycosidic bond. Thus on complete hydrolysis, it yields only one type of monomer i.e alpha-glucose monomers
7-d) are phosphorylated on mannose residue
Lysosomes are known to have 50 hydrolytic enzymes( like peptidase, protease), many of which carry a mannose-6-phosphate tag due to which they transfer lysosome to trans Golgi
8-b) nucleosome
The nucleosome is a basic structural unit of chromatin. It provides the first level of DNA-packaging and gives beads-on-a-string structure.
9-a) epigenetics
Epigenetics refers to both heritable as well as non-heritable changes which are not caused by an alteration in DNA sequence. suppose a methyl group is added to part of the DNA molecule due to which certain genes are prevented from being expressed
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