1. cell You need to expand cells in culture for an upcoming suspension and mix i
ID: 142977 • Letter: 1
Question
1. cell You need to expand cells in culture for an upcoming suspension and mix it with 100uL of trypan blue for counting. After total number of cells needed for the three flasks? Do you have enough cels to counting your 5mL cell suspension, you seed each flask at this density? experiment. You remove a 100L sample from your What verage of 55.5 an a cells. You need to seed 3 T-75cm2 flasks with 1x10s cells each. (6 points) sed each las Thin films are placed in individual wells of 24-well plates. Isolated cells are to be seeded on the films at a density of 10,000 cells/cm2, What is the total number of cells needed to seed 10 24-well plates? (4 points)Explanation / Answer
Total volume of cell suspension is 5ml= 5000 µl
Total number of cells in 5ml of suspension is = 55.5*5000/100
= 2775 cells
Number of cells needed for one T-75 cm2 flask = 1*106
Total number of cells needed to plate all three T-75 cm2 flask = 3* 1*106 = 3*106
The cell suspension doesn’t have enough cells to plate in this T-75 cm2 flask with this density.
2. For a 24 well plate the surface area of each well is 2cm2.
Density of cells in 24 well plates = 10000cell/cm2
Total number of cells in one well = 20000 cells
Total number of cells in one 24 well plate = 24*20000 cells = 480000 cells
Total number of cells needed to seed ten 24 well plates= 10*480000 = 4800000 cells = 4.8*106cells
Related Questions
Navigate
Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.