Two pepti the peptide sample was completely degraded after four cycles of Edman

Question

Two pepti the peptide sample was completely degraded after four cycles of Edman degradation. In the second method, three florescent dansylamino acids were chloride followed by treatment with an acid. de samples of the same sequence were degraded using two different methods. In first method, . generated after treating the sample with dansyl- a. Predict one possible sequence for the peptide using its 3-letter abbreviation ie Gly-Gly When determining the identity of the N-terminus amino acid of a given peptide, what is the advantage or drawback of using dansyl-chloride versus Edman degradation? b.

Explanation / Answer

a. Leucine- enkephalin Tyr-Gly-Gly-Phe-Leu.

b. Edman method allows sequencing of proteins throughout the cycle, but dansyl chloride can be used only for single degradation. Dansyl chloride is easier and quicker compared to edman reaction.

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