14. If a breeder wanted to use a mitotic inhibitor (e.g., colchicine or oryzalin

Question

14. If a breeder wanted to use a mitotic inhibitor (e.g., colchicine or oryzalin) to create an allotetraploid (AABB) sunflower that combines the diploid genomes of common sunflower (AA) Helianthus annuus and silverleaf sunflower (BB) Helianthus argophyllus, which plant(s) would they double (be specific) and how would they produce this(these) plant(s)? Which plant part (pollen, root, seed, meristem of a young seedling, developing buds) could they treat? 15. If a breeder wanted to use crosses and unreduced gametes to create an allotetraploid (AABB) sunflower using Helianthus annuus (AA) and Helianthus argophyllus (BB), which cross or crosses would they make (be specific)?

Explanation / Answer

14. You can use colchicine at two stages to get the allotetraploid. you can double the plant with AB genotype. First you go for normal cross A×B. Then at zygote stage you treat with colchicine and get AABB

15. Use unredeuced gametes AA×BB

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