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Which of the following is sterile? a previously autoclaved flask of King\'s B ag

ID: 142807 • Letter: W

Question

Which of the following is sterile? a previously autoclaved flask of King's B agar to which you just added a stir bar I Choose l an unopened container of microfuge tubes with autoclave tape on it that has changed color [ Choose ] a clean flask from the glassware r Choose cabinet an inoculating loop that you I Choose flamed for 10 seconds and put down on the bench a bottle of King's B liquid that Choose you left open while labeling your tubes the barrel of a micropipettor that you've wiped with a tissue moistened with distilled water

Explanation / Answer

In the first question when you add a stir bar there is a chance of contamination.

Only the second one is sterile.

Clean flask is not sterile. Autoclave is required to sterile the flask.

When you put the inoculating loop down on bench contamination may come.

If you open the bottol in air bacterial contamination may occur. You should open it in a sterile aseptic place.

You should wipe the tissue moistened with 70% ethanol as it kills the bacterial population.

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