Lab 2. Enzymes Fall 2018 Name Exercise 1. Experimental Method and the Action of
ID: 142673 • Letter: L
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Lab 2. Enzymes Fall 2018 Name Exercise 1. Experimental Method and the Action of Catechol Oxidase 1. Label the diagrams with the name of the enzyme and substrate that are used in experiment one. Enzyme Substrate Product 2. List in order the solutions you will put into the test tubes. 3. Which tube(s) serve as control(s)? Explain, Please read the section on enzymes in Ch. 8 of Campbell Biology (8.4) then answer the following questions 1. Define a catalyst. 2, Does an enzyme change G for a reaction? Does an enzyme change EA? 3. Most enzymes work in a very narrow temperature range (only a few degrees-most of yours will stop working long before they reach 45 C). Taking into account what you know about the how a substrate fits into the active site of the enzyme (for example, figure 8.15) and what you have leamed in lecture about how the three-dimensional shape of an enzyme (which is a protein) is held together, speculate on what types of bonds are breaking as the enzyme moves to a temperature that is too high? Exercise 2. Inhibition of Catechol Oxidase Some inhibitors bind to the active site of the enzyme and never release from it, and once bound to inhibitor, the enzyme is non-functional. Such inhibitors are called suicide substrates. Other inhibitors bind to the enzyme reversibly, so they may stay bound for a fraction of a second, release, diffuse apart, and then the enzyme can bind something else, either a substrate molecule or another inhibitor. Think about the experiment we are running. and how the specific components are added (enzyme, substrate, and inhibitor) Page 1 of 16Explanation / Answer
Excersice=1
1. Enzyme= catecholase (catechol oxidase) ; Substate= catechol and oxygen ; Product= benzoquinone and water
2. First tube is control then reaming would be sample tubes
3. First tube consider as control.There was no enzyme present in the control tube and the test tube all other experimental parameter should be same as like sample tubes.
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