0 1 pts Question 3 Oct4 is an embryonic stem cell specific transcription factor.

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0 1 pts Question 3 Oct4 is an embryonic stem cell specific transcription factor. ESCs grown in petri dishes are fed media that contain basic fibroblast growth factor (bFGF) which keeps the ESCs undifferentiated. They examine Oct4 mRNA levels in human ESCs (hESCs), hESCs with exogenous miR-223 (micro RNA 223), hESCs with exogenous miR-223 that do not have bFGF in the media, and hESCs without bFGF but with miR-223 inhibitor If extra miR-223 is added to the cells, this leads to (MORE/LESS/THE SAME) Oct4 levels. We conclude this based on which time points? B Oct-4 expression in different times +bFGF 1.5 hESCs Exogenous miR-223 Exogenous-bFGF 1.0 I miR-223 inhibitor -bFGF 0.5 0.0 12h 72h 120h More... Less... The same... .due to the 12hr .due to the 72hr ....due to the 120hr

Explanation / Answer

1)If extra mir-223 adds to cell this will lead to more oct4 level because more expression of+bFGF,we concluded this at 120 hr.

2)this data shows that mir 223 is important to keep ESCs undifferentiated only in the absence of bFGF because in presence it differentiate.

3)If the genetic codon was overlapping than correct answer would be GG UAG

4)f CCA added to tRNA than it would be possible to coonect amino acid because both are capable of phosphodiester bond formation.

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