How can I find the surface-to-volume ratio of S. epidermidis and E. coli? QUESTI

Question

How can I find the surface-to-volume ratio of S. epidermidis and E. coli?

QUESTIONS What are some consequences of leavimg a stain on a bacterial smear too long (over-staining)? What are some consequences of not leaving a stain on a smear long enough (under-staining)? Choose a coccus and a bacillus from the organisms you observed and calculate their surface-to-volume ratios. Consider the coccus to be a perfect sphere and the bacillus to be a cylinder. Use the equations supplied. Cell Morphology Surface Area Volume Coccus SA 4ri? Bacillus r-radius, h-height, " 3 14 Surface-to-Volume Ratio of Sample Cells Cell Morphology Surface Area (um2) Volume (Hm3) Surface-to-Volume Ratio Organism nsider a coccus and a rod of equal volume a. Which is more likely to survive in a dry environment? Explain your answer. b. Which would be better adapted to a moist environment? Explain your answer 190 MICROBIOLOGY: Laboratory Theory & Application

Explanation / Answer

S.epidermidis is a cocci,so use the formula of surface area and volume of coccus to find out the same.

Then divide surface area by volume. and you get the answer.... i.e

surface-to-volume ratio=surface area of cocci/volume of cocci

B) For E.coli : It is a bacillus(rod shaped) so use the formula of bacillus in surface area and volume and then substitute their values i.e-

surface-to-volume ratio=surface area of bacillus/volume of bacillus

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