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which agent was the least nsing agent was the most effective at killing bacteria

ID: 141842 • Letter: W

Question

which agent was the least nsing agent was the most effective at killing bacteria and w the effective? undine 3 Compare your observations from question #2 with others at your bench. Didyou all a ncesyo cleansers were most or least effective? Explain what variables might eipid the same procedun (why are your results different from others at your bench when you all did the same procedure) 4 Some bacteria grew on your plates even after you washed your fingerti why do you think that some of the bacteria managed to survive the hand washing ps with the different cleansers. Some cleaning agents are bactericidal and others are bacteriostatic. What do these words mean? Which is better: a bactericidal agent or a bacteriostatic agent?

Explanation / Answer

3.chlorhexidine is a broad spectrum anti bacterial agent with bactericidal activity which is based on its residual activity. It acts on the cell membrane and results in denaturation of proteins only after contact after several hours. Therefore it is effective only on long exposure where as alcohols are more effective and quick in action resulting in protein coagulation of the micro Organism. This causes difference in various effective hand cleansing agent.

4.Most of the hand sanitizers we use are alcohol based. They have anti bacterial activity by causing denaturation of proteins. This bacterio static action was believed to be caused by inhibition of the production of metabolites essential for rapid cell division. However alcohol destroys dehydrogenase enzymes and increase the lag phase of the Organism that could be reversed by adding certain amino acids, because of this reason, micro organisms are becoming increasingly resistant to this hand cleansers. Example-the multidrug resistant bacterium that is Enterococcus faecium can survive on hands even after hand wash.

5.Bacteriostatic agents are the meant to prevent the growth of bacteria i.e it keeps them in the stationary phase of the growth. Some of them are chloramphenicol, fusidic acid, tetracycline, macrolides, clindamycin, sulfamethoxazole.

Bactericidal agents are meant to kill the growth of bacteria. Some of them are beta-lactum, penicillin, cephalosporins, minibactams, vancomycin.