Question 1 2 pts Which of the following techniques is appropriate for purifying

Question

Question 1 2 pts Which of the following techniques is appropriate for purifying proteins from a complex solution following cell lysis? a) Atomic force chromatography, Electrophoretic centrifugation O b) Electron density crystallography, pH drift size exclusion O c) Size exclusion chromatography, ion exchange chromatography d) Differential centrifugation, asymmetric electrophoresis Question 2 2 pts Which of the following process best describes the polymerization of amino acids? O a) lon exchange between non-polar interactions O b) Van der walls interaction between R-groups O c) The formation of covalent bonds O d) The hydrolysis of amino acids

Explanation / Answer

Question 1

a) Size exclusion chromatography and ion exchange chromatography is best suited technique to separate proteins based in a complex solution. Generally proteins are separeted well based on thier size and affinity.

Question 2

d) The hydrolysis of aminoaicd is an indication of polymerisation of aminoacids. Polymerisation is a reaction between two aminoacids linked by a peptide bond, breaking the peptide bond releases the water molecule called as hydrolyis.

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