A protein containing several proline residues is: not likely to form quarternary
ID: 141310 • Letter: A
Question
A protein containing several proline residues is: not likely to form quarternary structures. not likely to form alpha helices. Which of the following statements is NOT correct regarding protein separation techniques? Specific antibodies are required for immunoblotting, antibody affinity chromatography as well as immunoprecipitation. Western blotting is a useful technique to isolate proteins for use in downstream assays. Which of the following statements about enzymes is FALSE? The Michaelis constant is an inherent property of an enzyme equal to the substrate concentration that yields maximal reaction rate. The maximal velocity of an enzymatic reaction is dependent on the concentration of enzyme. A protein containing several proline residues is: not likely to form quarternary structures. not likely to form alpha helices.Explanation / Answer
1. Answer: A protein containing several proline residues is not likely to form alpha helices
Explanation: Proline is known as helix breaker. t can not fit into the alpha helix due to its rigid imino group.
2. Answer: Western blotting is a useful technique to isolate proteins for use in downstream assays.
Explanation: In Western blotting, proteins are run on a gel, transferred to a membrane and probed with an antibody. It is an irreversible process. The native protein can not be re-obtained.
3. Answer: The Michaelis constant is an inherent property of an enzyme equal to the substrate concentration that yields maximal reaction rate.
Explanation: Km is the substrate concntration at which Vmax is half.
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