A. B. C. DNA Polymerase I DNA Polymerase III (core) Single strand binding 2-3 co

Question

A. B. C. DNA Polymerase I DNA Polymerase III (core) Single strand binding 2-3 copies occur in the functional unit Has 6 identical subunits Requires ATP Binds to the replication helicase Binds specific sequences of double-strand DNA Has 3' exonuclease activity Has 5' exonuclease activity protein (SSB) D. Primase (DnaG) E. Clamp (B subunit) F. t protein G. DnaA H. Replication Helicase (DnaB) I. Clamp loader (y complex) Requires all 4 types of ribonucleotide triphosphates Component of DNA polymerase III holoenzyme Makes and seals single-strand breaks in DNA Binds to the clamp loader Topoisomerase I K. DNA gyrase L. DNA ligase M. Dam methylase Binds non-specific sequences of single-strand DNA Requires deoxyribonucleotide triphosphates Drives the movement of the replication fork Interacts with the clamp Introduces negative supercoils Closes single-strand breaks in DNA Opens the duplex DNA at the origin of replication

Explanation / Answer

A. DNA polymerase 1 - had 5' exonuclease activity

B. DNA polymerase 3 - has 3' exonuclease activuty.

C. Single strand binding proteins - binds to specific sequences of single stranded DNA

D. Primase - requires all four types of ribonucleotide triphosphates

E. Clamp - component of DNA polymerase 3 holoenzyme

F. Sigma protein - has 6 identical subunit

G. DnaA - opens the duplex DBA at the origin of replication

H. DNA helicase - drives the movement of replication fork

I. Clamp loader - interacts with clamp

J. Topoisomerase 1 - makes and seals single strand breaks in DNA

K. DNA grade - requires ATP; introduces negative supercoil.

L. DNA ligase - closes single-strand breaks in DNA

M. Dam methylase - 2-3 copies in the functional subunit

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