why ar the othev Options wYDnG 21 You are growing a population of cells that app
ID: 141215 • Letter: W
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why ar the othev Options wYDnG 21 You are growing a population of cells that appear to be resistant to G418, a chemical that is normally toxic to cells. Upon further analysis of the cells' genome you find that it contains a genomic sequence that encodes the protein neomycin phosphotransferase. What would you do to test if this protein confers resistance to the G418 chemical? a. determine if the cells are also making -galactosidase b. tag the protein with green fluorescent protein to see if it is degraded by G418 c. label a fragment of the gene to see where it is expressed clone the gene into a G418-sensitive cell line, then treat these cells with G418Explanation / Answer
D. clone the gene into a G418-sensitive cell line, then treat the cells with G418
This seems the most appropriate answer to me. If we clone the gene for the protein neomycin phosphotransferase into a G418 sensitive cell line and treat the cells with G418 to see that the cells are not affected by G418, we can infer that this protein confers resistance against G418 to the cell line that previous to cloning was senstitive to G418.
The other options like production of beta-galactosidase, degradation of the neomycin protein and other may tell us about the general characteristic of the protein interaction with G418 or the cell behaviour but will not specifically conclude that neomycin phosphotransferase confers resistance against G418. While the other options tell us about the interactions and behaviour of the protein, it may not be concluded that this protein will confer any resistance as there may be some other cellular property confering resistance against G418.
But for option D, we will be sure if the desired protein confers any resistance against G418 or not.
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