7. Being a somewhat old-fashioned molecular biologist who likes do to your own e

Question

7. Being a somewhat old-fashioned molecular biologist who likes do to your own experiments when no one in the lab is watching, you decide to do a different types of genome analysis with the tardigrade genome. You isolate DNA from a tardigrade species and shear it into random fragments, with an average size of 1 kb. The fragmented DNA is heated to denature it to single-strands and is then allowed to reanneal by lowering the temperature. The percentage of the DNA that remains single-stranded is assayed at various times and plotted on a graph as shown below. Three components of the genome are observed. “Cot” on the X axis is a composite of the concentration at time 0 and the time, so this type of experiment was called a Cot curve.

a. What is component A, and why does it become double-stranded so quickly?

b. If the tardigrades are like most other metazoans, which component of the genome will account for most of the transcripts found in Question 6, and why?

c. If you wanted to study the role of a particular transcription factor, its gene is likely to be found among which component?

Genetics- please answer in good detail

Explanation / Answer

a. Component A corresponds to the part of genome which comprises of highly repetitive DNA sequence and hence tends to re-anneal fastest.

b. Most of the transcripts of mRNA should be contributed by Component C as most genes are unique in their sequence and hence such DNA stretches will take longer to renature. On the other hand, most transcripts of tRNA and rRNA should be present in Component B as they will be comparatively less unique.

c. It's gene will be found in Component C as it will be highly unique.

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