ou do not need to go to the external link as I have included the table informati

Question

ou do not need to go to the external link as I have included the table information blow the picture of the uestion. Suppose you isolate plasmid puci9 from E coll strain NM494 and then you transform the isolated plasmid into E coli strain Y10. Will you be able to successfully transform this pUC19 sample into Y102 Explain why or why not, Use the strain phenotype information found here https://www.neb.com/tools-and-reso urces/usage-guidel ines/mera-mcrbc-and-ecoki-strain- phenoypes NoTE bout the Table In the Strain Phenotype Table, the R" and Mt" headings refer to the EcoKI restriction system, which is encoded by the hsdRMS genes. "R refers to the hsdR gene, while "M" refers to the hsdM gene. + means the phenotype indicates normal gene activity (wild type), and "" means that the gene activity is absent. (3 pts) NM494McrA McrBC -EcoKR EcoKM pop101 Strain NM494 has non functioning McrA, functioning McrBC and non functioning EcokR and EcoKM. Y10+McrA +McrBC +ECOKR+ECoKM Strain y10 has functioning McrA, McrB, EcokR, and EcokM. External Link: https://www.neb.com/tools-and-resources/usage-guidelines/mcra-mcrbc-and-ecoki-strain phenotypes

Explanation / Answer

Yes, the plasmids fom one strain of E.coli to other srain can be performed but under certin conditions. The plasmids are prone to Dnase digestion . Hence pUC19 are entrapped into liposomes before transfer. These liposome proteced plasmids are transfected into the Y10 E.coli strain. Thus by use of liposome mediated transfection the number of transformants are more compared to transfection without liposome. This mode of transformation is highly efficient and convenient.

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