The same basic recombinant DNA technology process used to synthesize the TNF rec

Question

The same basic recombinant DNA technology process used to synthesize the TNF receptor for Enbrel was first used to synthesize large amounts of insulin in the laboratory in the 1980s. Complete and order the following statements to demonstrate your understanding of this process vector The insulin gene from a then amplified by PCR. chromosome is and insulin Restriction enzymes are used to cut open a plasmid for use as a cloning DNA ligases The insulin gene is inserted into the into its circular form. , and it is closed up again genetically modified human The plasmids are inserted into a host cell by bacterial transformation Bacterial cells begin to produce medium. when introduced to a culture conjugation plasmid The insulin is collected and for human use purified solated Reset

Explanation / Answer

1. a-human ( gene is present on human chromosome)

b-isolated ( should have to be isolated so onl that gene will get amplified)

2.a-restriction enzymes (make cut at a particular site in the bacterial plasmid)

b- DNA ligases ( both cut ends now joined by the help of DNA ligases.)

3- plasmid

4- a- bacterial

b- vector ( it helps to transport plasmid)

5- insulin ( when proper medium provided the will begain to produce insulin as plasmid get multiplide).

6.purified ( the insulin sholud have to be purified before it have to release in the market.)

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