Using bioinformatic databases (NCBI) about DNA sequence for the hen ( Gallus gal

Question

Using bioinformatic databases (NCBI) about DNA sequence for the hen (Gallus gallus) egg-white (LYZ) lysozyme gene:

a. How many introns are in the LYZ (Gallus gallus) gene?

b. How many introns are in the LYZ (Gallus gallus) gene?

c. On which chromosome is the LYZ (Gallus gallus) gene located?

d. How many base pairs (b.p.) long is the LYZ (Gallus gallus) DNA gene sequence

e. What gene is located immediately upstream of the LYZ gene? (Select one: FRS2 or KEGG or CPM or CPSF6?)

f. How many bases long is the mRNA transcript generated by the LYZ gene?

Explanation / Answer

Go to NCBI website, in the database drop down, select 'gene' and type 'LYZ Gallus gallus' in the textbox.

Gene ID is 396218

From this page alone most of the information to the above questions can be obtained:

a. 3 introns (there are four exons which means there are three introns, this can be clarified in the genbank link which opens up the gene entry in the nucleotide database. under the mRNA annotation it shows the position of all exons)

b. 4 exons

c. Chromosome 1

d. 3688 bp (click on the gen bank link near go to nucleotide)

e. CPSF6 (a graphic shows all the genes present on chromosome 1 and their order)

f. 584 bp (In the gen bank link, where the entire gene is annotated, scroll down to the part with mRNA. Click on the transcript ID link which opens up another nucleotide entry specifically for the mRNA. Here you can find all the information about the mRNA encoded by these genes.

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