EXERCISE 3 REVIEW SECTION Microscopy Name Date Lab Section Time Review Questions

Question

EXERCISE 3 REVIEW SECTION Microscopy Name Date Lab Section Time Review Questions I. If the ocular lens is 10x, what would be the total magnification for the following objective lenses? a, 5× b. 17x c. 35x 2. What is the function of the iris diaphragm of the microscope? 3. If the diameter of the field of view is 5.6 mm at 40x, what would the diameter be at 80x? 4. What is the name of the thin glass plate placed on top of a specimen'? 5. The microscope that you use in lab is a(n) a compoand light microscope. b dissecting microscope. c. electron microscope. 6. What is the name of the circle you see when you look through the ocular lens of the microscope? 7. When you switch from a low power objective lens for example, 4x) to a higher-power objective lens (for eanple, iox, wha happens to the working distance between the lens and the coverslip 8. What happens to the ficld of view when you change from a low-power objective lens to a higher-power objective lens? Does it increase or decrease 9. When should you use the scanning lens on the microscope? 10, How should you clean the lenses of microscope? 11. What is the proper way to carry the microscope in lab?

Explanation / Answer

1a. 50x, b. 170x & c. 350x. As the ocular lens is of 10x magnification, the objective lens magnification should be multiplied, hence 10x times of 5x in first part of question is 50x and so on.

2. Iris Diaphragm controls the amount of light reaching the specimen in light microscope or compound microscope. It is located above the condenser and below the stage of microscope.

3. The diameter would be 2.8 m.m. As the magnification doubles, the diameter would be 5.6 divided by 2 or 2.8m.m.

4. Cover slip is a thin glass plate of different shape normally placed over the top of a specimen.

5. Compound light microscope is normally used in lab.

6. It is called the field of view. It is normally designated by its diameter.

7. The actual working distance is decreased in this case.

8. The field of view increaseswhen a higher power objctive lens is used in compound light microscope.

9. Whenever a new slide is viewed or when the view of the specimen in the field of view of a higher-power lens is lost, then a scanning lens should be used.

10. A soft cleaning wipe should be used for cleaning the lenses of microscope.

11. A microscope should be carried with two hands. One hand should grasp the arm and the other should support it under the base,while keeping the lowest objective power lens popped at position of view.

13. a - 3.body tube

b - 7. objective lens

c - 9. stage

d - 5. iris diaphragm lever

e - 6. light source

f - 2. base

g - 8. ocular lens

h - 1. arm

i - 4. coarse focus knob

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