PAR T 3: MEASURING ABSORBANCE OF DIFFERENT DILUTIONS OF GREEN FOOD DYE Table 1 -
ID: 139984 • Letter: P
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PAR T 3: MEASURING ABSORBANCE OF DIFFERENT DILUTIONS OF GREEN FOOD DYE Table 1 - Take the absorbance of Solution A and use that measurement to predict th e absorbance of Solution B, C and D. Then measure their absorbances. Solution Concentration Predicted Absorbance Measured Solution A ·020 1.7% 3.3% 6.7% 13.3% 2b . 041 030 (measured) Solution B Solution C Solution D 052 . 104 200 D17 1. Which of these solutions had the most food coloring in it? How is this related to its absorbance reading? 2. Describe (using a sentence) one uncontrolled variable in this experiment. 3. Describe (using a sentence) one control variable built into the design of this experiment. PART 4: DETERMINING THE CONCENTRATION OF AN UNKNOWN DILUTION OF GREEN FOOD DYE 4. What was the absorbance of the unknown green dye sample? From your graph, what did you determine to be the concentration of the unknown green dye sample? Absorbance of unknown sample 009 Concentration of unknown sample What did you learn from this lab? 5. Lab 2 Methods Tools and Measurements Data Sheet-Page 3Explanation / Answer
Ans.1 Solution D has the most food colouring in it as it is clearly stated the concerntration of solution D is maximum (13.3%). It is related to absorbance reading as by increasing the concerntration, the reading of absorbance increases hand in hand. Beer's law relates to the concept of concentration and absorbance. According to this law, concentration and absorbance are directly proportional.
Ans.2 Absorbance will be uncontrolled variable in this experiment because it is dependent on other variables which can be controlled by you.
Ans.3 Concentration is the controlled variable which can be controlled during setting up the experiment.
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