Sec mutants can be divided into 5 distinct classes (A-E) based on where the GFP-

Question

Sec mutants can be divided into 5 distinct classes (A-E) based on where the GFP-secretory protein accumulates at the nonpermissive temperature. Indicate the Sec classes and N-linked Glycosylation state your would observe for a GFP-secretory protein if each of the following components were defective at the nonpermissive temperature. The superscript indicates the normal location of the particular component.
v-SNARE late endosome
A) Class D with NAG glycosylation
B) Class D with NANA glycosylation
C) Class E with NAG glycosylation
D) Class E with NANA glycosylation
E) Secreted with NANA glycosylation

Explanation / Answer

Sec mutants can be divided into 5 distinct classes (A-E) based on where the GFP-secretory protein accumulates at the nonpermissive temperature. Indicate the Sec classes and N-linked Glycosylation state your would observe for a GFP-secretory protein if each of the following components were defective at the nonpermissive temperature. The superscript indicates the normal location of the particular component.
v-SNARE late endosome
A) Class D with NAG glycosylation
B) Class D with NANA glycosylation
C) Class E with NAG glycosylation
D) Class E with NANA glycosylation
E) Secreted with NANA glycosylation

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