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please and thank you! Pure Culture Techniques (continued) 3. Subculture Evaluati

ID: 136097 • Letter: P

Question

please and thank you!

Pure Culture Techniques (continued) 3. Subculture Evaluation With colored pencils, sketch the appearance of the growth on the slant diagrams below. If you prepared and observed Gram-stained slides of the subcultured bacteria, draw a few cells of each organism as re- vealed by Gram staining in the adjacent circle Serratia marcescens (or Escherichia coli) Micrococcus luteus (or Chromobacterium violaceum) 4. Compare the results of your streak and pour plates. Which method achieved the best separation of species? 5. Do your slants contain pure cultures? How would you confirm their purities?

Explanation / Answer

Answer 4

pour plate method of counting bacteria is more precise than the streak plate method, but, on the average, it will give a lower count.The pour plates achieved better results

Answer 5

Yes they do, 1 slant contains serratia marcescens and 1 slant contains E.coli. To confirm this

take a sample of the slant and make a smear and gram stain cells and look under microscope.