Questions on DNA Synthesis and Gels Questions on Bacterial Growth Name theoe pym

Question

Questions on DNA Synthesis and Gels Questions on Bacterial Growth Name theoe pymimidioe hases, and what purine hases do they bave pair w.ith 1. When growing Ecoh in liquid medium, why do we shake the flask while growing the cells in the incubator? 2 When a DNA chromosome has heen replicated, there are now two DNA molecules and they are hoth double stranded Where are the two old strands of DNA? 2 Why do bacteria stop growing during the stationary phase of the growth curve? 1 Daring DNA replication a deoxy-nucleotide is added to the grouing strand of DNA To which carbon ato on the deoxynihose is the new nucleotide added? 3 If E coll grows faster at 37-C than they do at 20-C would they grow better at What does O.D.(optical density) mean? 4 If you loaded your DNA sample to a gel and the well was at the top of the gel, at which end of the gel should the negative electrode be connected? 5. Since C botulinum will grow in the presence of O authors grow C botulinum anaertically? or in its absence, why did the Why do large fragments of DNA run sower through a gel?

Explanation / Answer

Ans1... pyrimidine bases...Thymine,cytosine ,uracil

Purine bases...adenine and guanine

Thymine and uracil pair with adenine

Cytosine pair with guanine

Ans 2...the two old strands of DNA is in the both new strands.one old strand is in the one new DNA and other old strand is the other new DNA.

Ans 3...to.carbon atom number 3 a deoxynucleotide base is added during the replication of DNA

Ans 4...DNA is negatively charged so it will be attracted towards positive charge so positive charge electrode should be away from well and negative charge electrode should be towards the well end

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