uw 3 Questions from Prokaryotic Transcription Eukaryotic Transcription Questions
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uw 3 Questions from Prokaryotic Transcription Eukaryotic Transcription Questions The two main bacterial promoter cis elements (TATAAT and TTACA) are located 1. What kind of RNA does RNA polymerase III transcribe where with respect to the tramcription start site? 2. What does a sigma factor do for a bacterial RNA polymerase? 2. A gene has an upstream sequence at -25 that reads, TAAAT This is expeessed at a low level. You introduce a nuclootide change at-25 to change the soquence to, "TATAT What changs, if any, would you expect in this gene's twanscription level, Le. is there more or less mRNA made? 3. If a repressor prodein binds to the promoter region of the lac operon, transcription of that mRNA cannot occur. How does the repressor prevent transcription? 3. Afer eukary etic tancription is initiased, de THID, TFIIB, ec maistain centact with the RNA polymerase as it progresses down the DNA strand 4. If you incubated an E.coli culture for several hours in a medium containing lactose and glucose as the only carbon sources (sources of nitrogen, phosphates, and trace elements would be included), the bacteria would grow fine. If you tesied the solution after a few hours of incubation, which of the two carbon sources would be in high or low Decs an cohancer tell the RNA Pelymerase lI where te start transcription When does the CAP protein bind to its recognition site near the promoter of a bacterial gene? 5. s. What pretein is an essential subuanit in ene of the general transcriptien facters of all three eukaryotic RNA pelymerases?Explanation / Answer
Prokaryotic transcription
1)TATAAT (the TATA box) is located at -10 i.e. 10 base pairs upstream of the transcription start site.
2)TTGACA is located at -35 i.e. 35 base pairs upstream of the transcription start site.
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