The top strand of the following segment of DNA serves as the template strand. We

Question

The top strand of the following segment of DNA serves as the template strand. We will refer to this segment of DNA as the original (or unmutated) sequence.:

3’ TACACCTTGGCGACGACT 5’

5’ ATGTGGAACCGCTGCTGA 3’

The original (unmutated) DNA sequence (above) has been mutated to the following (this represents the template strand):

3’ TACATCTTGGCGACGACT 5’. <------------- We will refer to this sequence as mutation #1.

*Please note that for simplicity only the template strand for this mutated segment of DNA is shown.

Answer the following questions:

(a) What is the complete mRNA sequence for the mutated segment mutation #1?

The mutated mRNA sequence is: 5' ________________________ 3'.

**Please enter your sequence in the 5' to 3' direction.**

(b) Using the mRNA sequence you determined in part (a) of this question, give the sequence of the protein that would be translated.

The amino acid sequence for this protein is N-terminus __________________________ C-terminus.

**Please note**

The N-terminus refers to the beginning of the primary sequence for a protein, and the C-terminus refers to the end of the primary sequence for a protein.

i.e. input the amino acids in the order that they would be translated.

If a codon encodes for a stop codon, type STOP.

When inputting your sequence, separate each amino acid with a hyphen (e.g. Ser-Tyr-STOP).

You will need to consult the genetic code to answer this question.

Explanation / Answer

Ans)

1. DNA: TACACCTTGGCGACGAT

   m RNA: 5' AUGUGGAACCGCUGCUGA 3'

2. 3 nitrogen bases of a m RNA nucleotides is called a codon. Each codon codes for one amino acids during translation

AUG UGG AAC CGC UGC UGA

Met (the strart codon) - trp - asn - arg - cys - stop

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