How would you examine the temporal and spatial patterns of expression after indu

Question

How would you examine the temporal and spatial patterns of expression after induction by low temperature?

Answer should been translational fusion with GFP gene and translational fusion with lacZ gene, because the question was asking about the observation, but Mastering genetic responded as incorrect!!!!!!!!!!!!!!!!!!!!!! And transcriptional fusion with lacZ gene and transcriptional fusion with lacZ gene is also incorrect!!!!!!!!!

If you could add an explantion with the correct answer, I would greatly appriciate it.

The CBF genes of Arabidopsis are induced by exposure of the plants to low temperature. Part A How would you examine the temporal and spatial patterns of expression after induction by low temperature? Select the two correct answers. translational fusion with GFP gene transcriptional fusion with lacZ gene translational fusion with lacZ gene transcriptional fusion with GFP gene Submit Previous Answers Request Answer

Explanation / Answer

the answer would be transcriptional fusion with lacz and translational fusion with GFP,

the transcriptional fusion with lacZ allows to study the function of an inducible promoter

And the proteins tagged with GFP gives an idea of the amount of translated protein. measured using FRET fluorescence resonance energy transfer.

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