4. If you have 77 colonies on a plate and did the following dilutions, how many
ID: 134794 • Letter: 4
Question
4. If you have 77 colonies on a plate and did the following dilutions, how many colonies per ml were in the original culture? Write the individual dilution, total dilution, and CFU/ml next to each tube or petri plate. (10 points) 1 ml 1 ml 1 ml 1 ml ml 9 m 9 ml 9 ml 0.1 ml 7 colonie 5. If you have 214 colonies on a plate and did the following dilutions, how many colonies per ml were in the original culture? Write the individual dilution, total dilution, and CFU/ml next to each tube or petri plate. (10 points) 1 ml 1 ml 1 ml 1 ml ture ml 9 ml 99 ml 99 m 1 ml 14 colonieExplanation / Answer
Original cultute-------------- Tube 1 (100 times dilution) --------------Tube 2 (10 times dilution) -----------------Tube 3 (10 times dilution) --------------- Tube 4 (100 times dilution)
Total dilution: 1000000 times dilution
Original culture: 7.7 X 108 CFU/ml
Tube 1: 7.7 X 106 CFU/ml
Tube 2: 7.7 X 105 CFU/ml
Tube 3: 7.7 X 104 CFU/ml
Tube 4: 770 CFU/ml
Original cultute-------------- Tube 1 (10 times dilution) --------------Tube 2 (10 times dilution) -----------------Tube 3 (100 times dilution) --------------- Tube 4 (100 times dilution)
Total dilution: 1000000 times dilution
Original culture: 2.16 X 108 CFU/ml
Tube 1: 2.14 X 107 CFU/ml
Tube 2: 2.14 X 106 CFU/ml
Tube 3: 2.14 X 104 CFU/ml
Tube 4: 214 CFU/ml
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