n euharyotes there ave large epetitive D.Nal segments in the geneme. These large
ID: 134661 • Letter: N
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n euharyotes there ave large epetitive D.Nal segments in the geneme. These large vepetitive DNl segments pevented unambiguous assembly of contigs into a uhole geneme. Shis proßlem was everceme by the useef Single-end veads lgnueoides Molecular markens Taired-end veads a veference genome is the best sepresentation of a geneme of an erganism, Eut the reference genome has inaccuracies and gaps. epvesentatian a+ a genane anganiom, J sue False Jnc«asing the and detection af stuuctuval variants can improve the de neve geneme assembly Sequencing speed DNl cencentvation Read length c RNdcencentrationExplanation / Answer
In Eukaryotes there are large repititive DNA segments in the genome.These large repititive DNA segments presented unambiguous assembly of contigs into a whole genome.This problem was overcome by the use of Paired end reads.
The term paired ends refers to the two ends of the same DNA molecule.The two sequence you get are paired end reads called 'mate pair' or 'paired end'.
Genome aseembly still remains a challenge despite the computational advances.In particular,the abundance og repeat elements in genome makes it difficult to assemble them into a single complete sequence.Mate pair information can be used complimentarily for genome assembly.
A reference genome is best representation of genome of an organism,but the reference genome has inaccuracies and gaps-TRUE
A reference genome is a digital nucleic acid sequence database,assembled by scientists as a representative example of species set of genes.
As the cost of DNA sequencing falls,and new full genome sequencing technologies emerge,more genome sequences continue to be generated.Reference genomes are typically used as the guide on which new genomes are built,enabling them to be assembled much more quickly and cheaply than the initial Human Genome Project.Most individuals with their entire genome sequenced,had their genome assembled in this manner.For much of the genome,the reference provides a good approximation of the DNA of any single individual.But in regions with high allelic diversity,such as major histocompatility complex in humans and the major urinary proteins of mice,the reference genome may differ significantly from other individuals.comparison between the reference and Watson's genome revealed 3.3 million single nucleotide polymorphism differences,while about 1.4 percent of his DNA could not matched to the reference genome at all.
Incraeasing the Read length can imorove the de novo genome assembly and detection of structural variants.
Genome assembly refers to the process of taking large number of short DNA sequences and putting them back together to create a representation of the original chromosomes from which the DNA originated.De novo genome assemblies assume no prior knowledge of the source DNA sequence length,layout or composition.
Long read technology to complement shorter reads provides accurate,complete characterization of any species.Increasing raed length is viewed as a crucial condition to further improve the assembly.
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